Yeast lytic enzyme used for digestion of yeast and fungal cell walls.

• Specifications: Lyophilized enzyme provided with Zymolyase Storage buffer.
• Source: Arthrobacter luteus
• Activity: β-1,3-glucanase
• Essential Enzyme: β-1,3-glucan laminaripentaohydrolase
• Optimum pH and Temperature: pH 7.5, 35 °C (lysis of viable yeast), pH 6.5, 45 °C (hydrolysis of yeast glucan)
• Unit Definition: One unit (U) of lytic activity is defined as the amount of enzyme that catalyzes a 10% decrease in optical density at 800 nm (OD800) in 30 minutes
• Assay Condition: Yeast (0.8 - 1.0 OD800) in 50 mM potassium phosphate, pH 7.5, 10 mM 2-mercaptoethanol

Digestion of yeast and fungal cell walls is necessary for many experimental procedures including spheroplasting, immunofluorescence, transformation, protein purification, and others. The use of lytic enzymes like Zymolyase are routinely used for digestion. The Zymolyase from Zymo Research is prepared from Arthrobacter luteus and is 100T equivalent. The storage buffer provided with the lyophilized enzyme has been optimized to confer maximal levels of enzymatic activity. R-Zymolyase also contains RNase A.

Rnase A not included