About this item
This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm.
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Specifications
- Conjugation:DABCYL-EDANS
- Storage conditions:–20 °C
- Protein synonyms:RET S1|HCV protease substrate
- Protein/peptide name:HCV Protease Substrate
- Purity:95%
- Molecular weight:1548.6 Da
- Sequence:Ac-DE-D(Edans)-EE-Abu-ψ-[COO]-AS-K(Dabcyl)-NH3
Specifications
Supplier: BDH
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: Corning
Supplier: VWR International