Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/ca/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Specifications
- Description:1-Step™ NBT/BCIP Plus Suppressor Solution
- Size:100 mL
- Cat. no.:CAPI34070
- Application:Immunoblotting
- Supplier No.:34070
- No. of Uses:10 uses at 10 ml each
Specifications
About this item
The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
- Sensitive with low background and minimial assay-to-assay variability
- Ready-to-use single component
The combination of NBT and BCIP yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, an enzyme conjugate for antibody probes. Contains 1 mM levamisole to inhibit endogenous phosphatase activity, while yielding an intense dark purple precipitate in the presence of calf intestinal phosphatase. Used for immunoblotting, immunohistochemistry, and in situ hybridization applications.