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Specifications
- Sensitivity:80% B/B0: 29 pg/ml
- Brand name:Cayman
- Cat. no.:75816-930
- Contents:Kit contains: Antiserum, Tracer, Standard, Buffers, Polysorbate 20, Reagent, COX-2 (human recombinant), Heme, Arachidonic Acid (substrate), Potassium Hydroxide, Hydrochloric Acid, Stannous Chloride, and Plate
- Range:15.6 - 2,000 pg/mL
- Supplier No.:560131-96
Specifications
About this item
Complete drug discovery assay for screening COX-1 and COX-2 inhibitors
- Screen for inhibitors of ovine COX-1 or human COX-2
- Directly measure PGF2α produced in the COX reaction
- Assay 15 samples for COX-1 or COX-2 in duplicate
- Plate-based colorimetric measurement (405-420 nm)
- Midpoint: 50% B/B0: 125-250 pg/ml
The COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major PG compounds. Thus, the Cayman COX Assay is more accurate and reliable than an assay based on peroxidase inhibition. Cayman’s COX Inhibitor Screening Assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.