Competitive ELISA for quantitation of PGD2 in biological samples

• Treatment of samples with MOX hydrochloride converts unstable PGD2 to stable PGD2-MOX
• Highly sensitive
• Measure PGD2-MOX levels down to 3.1 pg/ml
• Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
• Midpoint: 50% B/B₀: 10-35 pg/ml

This PGD2-MOX ELISA is based on the conversion of PGD2 to a stable MOX derivative. Treatment of the sample with MOX HCl converts PGD2 into PGD2-MOX, preventing its further chemical degradation. The antiserum used in the assay was developed using conjugates of this derivative and is very specific for PGD2-MOX.