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Specifications
- Description:SensoLyte® 520 Cathepsin L Assay Kit *Fluorimetric*
- Size:1 Kit
- Storage temperature:-20°C
- Cat. no.:103010-644
- No. of Wells:96
- No. of Assays/Kit:100 assays
- Supplier No.:AS-72218
Specifications
About this item
Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases
Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities.
The cathepsin assay kits offers a series of fluorimetric long wavelength, sensitive SensoLyte® 520 assay kits for enzyme activity determination of Cathepsins G, D, E, B, K, L and S. These kits employ a FRET (Fluorescence Resonance Energy Transfer) peptide substrate that emits in the green range of 520 nm, with excitation at 490 nm. The advantage of using longer wavelength emitting substrates means that autofluorescence from cellular components and test compounds is minimal; thus making the kits more sensitive. Furthermore, the use of HiLyte Fluor™ 488 (except for Cathepsin S, which uses 5-FAM) as the FRET donor (fluorophore) results in a more stable fluorescent signal, since HiLyte Fluor™ 488 is resistant to acidic pH. A listing of the SensoLyte® Cathepsin Kits providing conventional FRET (Mca/Dnp) or fluorogenic substrates for cathepsins are also available.
Kits detect specific Cathepsin activities; provides ample reagents to perform 100 assays in 96-well format; minimal hands-on time required; detailed protocol and references provided.
It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins. The SensoLyte® 520 Cathepsin L Activity Assay Kit uses a QXL® 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measuring enzyme activity. The long wavelength FRET substrate is designed based on a sequence surrounding the cleavage site of Cathepsin L. The QXL® 520/HiLyte™ Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin L cleaves the FRET substrate, it results in an increase of HiLyte™ Fluor 488 fluorescence and is monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, the optimal pH for Cathepsin L activity.