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Specifications
- Description:SensoLyte® pNPP Alkaline Phosphatase Assay Kit *Colorimetric*
- Size:1 Kit
- Storage temperature:4 °C
- Cat. no.:103010-494
- No. of Wells:96
- No. of Assays/Kit:500 assays
- Supplier No.:AS-72146
Specifications
About this item
Alkaline phosphatase is a popular enzyme conjugated with secondary antibody for ELISA
- Features: Kits include all essential components (chromogenic/fluorogenic/luminometry substrates) and sample reagents needed to perform 500 assays using 96-well plate format.
- Optimized to detect specific alkaline phosphatase activity; compatible for HTS; detailed protocol and references provided.
Phosphatases are important classes of enzymes involved in cellular signal transduction, cell metabolism and other biological activities. Changes in alkaline phosphatase level and activity are involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation related diseases, inflammatory bowel disease, post-parathyroidectomy stage and drug toxicity. Phosphatases have attracted considerable interest as: Enzyme markers, Drug screening targets, Conjugating agents and Reporter for gene expression studies.
p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for alkaline phosphate. This SensoLyte® kit can be used to detect alkaline phosphatase activity in biological samples and alkaline phosphatase-based ELISA.