About this item
Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.
- Easy to use
- Convenient and saves storage space
- Purified protein provides fewer chances of cross reaction with assay components
This 10% (w/v) solution of high quality BSA that is useful for saturating excess protein binding sites on membranes and microplates in immunoassays. Blocker BSA Buffers are most frequently diluted 10-fold (to 1% BSA) in 1X Tris-buffered saline for initial testing, but other buffer concentrations can be beneficial for specific systems. Blocker BSA is usually more effective than non fat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid endogenous biotin.
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