Pierce™ SulfoLink™ Immobilization Kit for Peptides, Thermo Scientific
Catalog # CAPI44999
Supplier: Thermo Scientific
CAS Number:
Specifications
- Description:SulfoLink™ Immobilization Kit for Peptides
- Size:5 x 2 ml
- Cat. no.:CAPI44999
- Supplier No.:44999
- Sufficient For:Immobilize 1 - 10 mg of antibody per column
Specifications
About this item
Immobilization Kit for Peptides, SulfoLink™ is a kit containing all the reagents for preparing the peptide sample for efficient immobilization and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.
- Specific to sulfhydryl (-SH) groups
- Prepare and couple protein samples in two hours
- Flexible coupling conditions, use pH 7.5 to 9.0 aqueous buffers, organic solvent or denaturant
- Streamlined protocols for sample preparation, immobilisation, and affinity purification
- Immobilise 1 to 2 mg of peptide per 2 mL column of SulfoLink™ Coupling Resin
When incubated with a solution of peptide that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.
Delivery: Kit include SulfoLink™ columns, 2 mL (5 columns), SulfoLink™ coupling buffer (120 mL), wash solution (120 mL), PBS pack (makes 500 mL), Bond-Breaker TCEP solution, neutral pH (0,5 mL), L-Cysteine·HCl (100 mg), and column accessories.