The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes

• Fast assay with femtogram levels of detection in about 1 hour
• No expensive hardware or vacuum required; no clogging issues
• Optimised protocol makes Western blot analysis easier
• Complete kits contain all components needed to block, probe and develop a blot

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classical Western blotting with SuperSignal West Dura Chemiluminescent Substrate. Like other Western blotting methods, the included SuperSignal West Dura Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse primary antibody.

The kit provides all the reagents necessary to complete a Western blot that was probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting. This kit includes SuperSignal™ West Femto Chemiluminescent Substrate, a maximum sensitivity HRP substrate that is detected using photographic or other imaging methods. This substrate produces a signal that is stable for several hours, enabling repeated film exposure to obtain optimal results.