Highly sensitive ELISA assays require minimal non-specific interactions

This product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X TBS Fish Gel Concentrate consists of 1.0 M Tris hydrochloride, 1.5 M sodium chloride and Fish Gelatin at pH 7.5. A proprietary combination of stabilizers and preservatives are used that are azide and mercury free.

Non-specific binding results in elevated background levels and a decrease in signal-to-noise ratios. Blocking reagents act to minimize non-specific interactions of secondary reactants with each other and with the primary solid phase binding sites.

This product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X TBS Fish Gel Concentrate consists of 1.0 M Tris hydrochloride, 1.5 M sodium chloride and Fish Gelatin at pH 7.5. A proprietary combination of stabilizers and preservatives are used that are azide and mercury free.

This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Storage Condition: Store container at 4° C before opening. Protect from moisture and light. No special shipping conditions or precautions are required.