You searched for: Protein Linking and Labeling Systems

Supplier: Thermo Scientific

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Supplier: Thermo Scientific

Thermo Scientific Pierce™ PEGn-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a n-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.

Supplier: Thermo Scientific

Pierce™ FITC Antibody Labeling Kit is a multi-component kit containing a high performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Supplier: New England Biolabs (NEB)

CLIP-Cell™ 505 is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro

Supplier: New England Biolabs (NEB)

SNAP-Surface® 594 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living or fixed cells

Supplier: G-Biosciences

G-Biosciences' recombinant streptavidin comes from streptomyces avidinii, produced in E.coli

Supplier: New England Biolabs (NEB)

SNAP-Cell 647-SiR is a far-red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro

Supplier: New England Biolabs (NEB)

SNAP-Cell® Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag® in solution or in living cells

Supplier: New England Biolabs (NEB)

SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells

Supplier: New England Biolabs (NEB)

The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the target protein from the affinity tag.

Supplier: New England Biolabs (NEB)

A 52.8 kDa non-glycosylated protein with a very high affinity for biotin

Supplier: Thermo Scientific

Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.

Supplier: Thermo Scientific

Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

Supplier: Thermo Scientific

The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 - 145 nm in excitation/emission wavelengths.

Supplier: G-Biosciences

G-Biosciences' femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining

Supplier: G-Biosciences

G-Biosciences' amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups

Supplier: Thermo Scientific

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions

Supplier: Thermo Scientific

An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

Supplier: Thermo Scientific

The carboxy-PEG_n-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers

Supplier: New England Biolabs (NEB)

CLIP-Biotin is a cell-permeable substrate (BC-Biotin) based on biotin with an amidocaproyl linker

Supplier: Thermo Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

Supplier: Thermo Scientific

DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.