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Specifications
- Cat. no.:75817-230
- Range:5 - 35 µm
- Includes:Kit contains: Standards, Buffer, Reagents, and 96-Well Plates
- Supplier No.:780001-192
Specifications
About this item
For the measurement of total nitrate/nitrite concentrations.
- Measure nitrate, nitrite, and/or total nitrate/nitrites in plasma/serum, urine, tissue culture media, and tissue homogenates
- Assay 40 samples in duplicate/96-well plate
- Assay Range: 5-35 µM
- Plate-based colorimetric measurement (540-550 nm)
The Cayman Chemical Nitrate/Nitrite Assay Kit provides an accurate and convenient method for measurement of total nitrate/nitrite concentration in a simple two-step process
This kit can be used to measure nitrate and nitrite in plasma/serum, urine, tissue culture media, and tissue homogenates. However, it cannot be used to analyze nitrate and nitrite from an in vitro assay of nitric oxide synthase (NOS) in which excess NADPH has been added. For these assays a second method (LDH method) is utilized and is available from Cayman Chemical in a 96 well plate format.
The first step is the conversion of nitrate to nitrite utilizing nitrate reductase. The second step is the addition of the Griess Reagents which convert nitrite into a deep purple azo compound. Photometric measurement of the absorbance due to this azo chromophore accurately determines nitrite concentration.