For quantitative fluorometric determination of alkaline phosphatase (ALP) activity.

• Detection limit of 0.02 U/L
• Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 20 minutes
• All reagents are compatible with high-throughput liquid handling instruments

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready. Procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery. This improved method utilizes 4-methylumbelliferyl phosphate that is hydrolyzed by ALP into a highly fluorescent product 4-methylumbelliferone. The rate of the fluorescence increase is directly proportional to the enzyme activity.