Specifications
- Description:Xanthine Oxidase Assay Kit
- Size:100 assays
- Method:OD570 nm, FL530/585 nm
- Sample:Cell lysate, serum, and other biological samples.
- Processing time:20 minutes
- Cat. no.:75878-180
- Supplier No.:EXOX-100
Specifications
About this item
For quantitative determination of xanthine oxidase enzyme activity.
- Linear detection range 0.03 - 25 U/L xanthine oxidase for colorimetric assays and 0.01 -2.5 U/L for fluorimetric assays
- Convenient room temperature and high-throughput assay
Xanthine oxidase catalyzes the oxidation of xanthine to uric acid
BioAssay Systems’ xanthine oxidase assay kit uses a single Working Reagent that combines the xanthine oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to xanthine oxidase activity in the sample.
In addition, xanthine oxidase can catalyze the oxidation of hypoxanthine to xanthine, act on certain purines and aldehydes, and in certain cases produce the superoxide ion. Clinically, xanthine oxidase activity in blood can act as a marker for influenza, liver damage, and possibly cardiovascular health. Simple, direct and high-throughput assays for measuring xanthine oxidase activity find wide applications in research and drug discovery.