For quantitative determination of nitric oxide synthase (NOS) activity.

• Detection range 0.25 - 25 U/L in 96-well plate
• Rapid and reliable

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions

BioAssay Systems’ EnzyChrom™ Nitric Oxide Synthase Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 40 min.

NO, an oxidation product of arginine by nitric oxide synthase (NOS), is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Simple, direct and non-radioactive. Procedures for measuring NOS are becoming popular in Research and Drug Discovery.