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Specifications
- Description:Malate Assay Kit
- Size:100 assays
- Method:OD565 nm
- Sample:Malate in food, juice, beverage and other agricultural products.
- Processing time:15 minutes
- Cat. no.:75878-116
- Supplier No.:EMAL-100
Specifications
About this item
For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism.
- Linear detection range 0.02 to 2 mM L-malate.
- Convenient room temperature assay
- No 37°C heater is needed
- Can readily automated to test thousands of samples per day
L-malate , or L-Malic acid, is a dicarboxylic acid that is made by all living organisms and plays an important role in the Calvin and Krebs Cycle
BioAssay Systems’ L-malate assay kit is based on malate dehydrogenase catalyzed oxidation of malate in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm is proportional to the malate concentration in the sample.
It is a source of CO2 for the Calvin cycle in plants and is also an intermediate that forms from fumarate in the Krebs Cycle. Malate is frequently used in food and beverage industries as an additive in products such as wine, beer, candies, etc.