For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism.

• Linear detection range colorimetric assay 5 - 1000 μM, fluorimetric assay 3 - 100 μM CoA
• Convenient room temperature "mix-and-read" assay
• Can be readily automated to process thousands of samples per day

Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others

BioAssay Systems’ method provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H₂O₂. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the CoA concentration in the sample.

One of CoA’s most crucial roles is the carrying and transferring of acyl groups.