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Specifications
- Description:Isocitrate Assay Kit
- Size:100 assays
- Method:OD565 nm
- Sample:Food, beverage, biological Samples (e.g. cell lysate, tissue homogenate, serum, etc.).
- Processing time:10 minutes
- Cat. no.:75878-066
- Supplier No.:ECIC-100
Specifications
About this item
For quantitative colorimetric determination of isocitrate and evaluation of drug effects on its metabolism.
- Linear detection range 20 to 5000 μM for 10 min reaction
- Homogeneous "mix-incubate-measure" type assay Can be readily automated to process thousands of samples per day
Isocitrate (Isocitric acid) is a substrate in the citric acid (TCA) cycle
BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.
Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.