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Specifications
- Description:Choline Assay Kit
- Size:100 assays
- Method:OD570 nm, or FL530/585 nm
- Sample:Serum, plasma, urine, saliva, milk, tissue, cells etc.
- Processing time:30 minutes
- Cat. no.:75878-064
- Supplier No.:ECHO-100
Specifications
About this item
For quantitative determination of choline and evaluation of drug effects on choline metabolism.
- Linear detection range colorimetric assay 1 to 100 μM, fluorimetric assay 0.2 to 10 μM choline
Choline and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission
BioAssay Systems’ method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to βine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.
Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety.