For sensitive colorimetric determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.

• Linear detection range 0.05 - 10 μM NAD⁺/NADH in 96-well plate assay
• Can be readily automated to process thousands of samples per day

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels

BioAssay Systems’ EnzyChrom™ NAD⁺/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD⁺/NADH concentration in the sample. This assay is highly specific for NAD⁺/NADH and with minimal interference (<1%) by NADP⁺/NADPH. Our assay is a convenient method to measure NAD, NADH and their ratio.

Quantitative determination of NAD⁺/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready. Procedures for measuring NAD⁺/NADH concentration are very desirable.