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- Description:Glutathione Assay Kit
- Size:250 assays
- Method:OD412nm
- Sample:Whole blood, plasma, serum, urine, tissue and cell extracts.
- Processing time:30 minutes
- Cat. no.:75877-994
- Supplier No.:DIGT-250
For quantitative determination of reduced glutathione (GSH) and evaluation of drug effects on its metabolism.
- Linear detection range 0.4 - 100 μM in 96-well plate
- Low interference
- Amino acids and common buffers do not interfere
Glutathione is a tripeptide of glycine, glutamic acid and cysteine
BioAssay Systems’ QuantiChrom™ Glutathione Assay Kit is designed to accurately measure reduced glutathione in biological samples. The improved 5,5’-dithiobis(2-nitrobenzoic acid (DTNB) method combines deproteination and detection (Reagent A) into one reagent. DTNB reacts with reduced glutathione to form a yellow product. The optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample. The optimized formulation has a long. Shelf Life: and is completely free of interference due to sample turbidity.
In the red blood cell, the reduced form of glutathione is vital in maintaining hemoglobin in a reduced state and hence protecting the cells from oxidative damage. Glutathione is involved in detoxification of hydrogen peroxide through glutathione oxidase. Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6-phosphate dehydrogenase, glutathione synthase and glutathione reductase. Simple, direct and automation-ready. Procedures for measuring reduced glutathione are becoming popular in Research and Drug Discovery.