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Specifications
- Description:Albumin (BCP) Assay Kit
- Size:250 assays
- Method:OD610 nm (BCP)
- Sample:Serum, plasma, urine, biological preparations
- Processing time:Five minutes
- Cat. no.:75877-982
- Supplier No.:DIAP-250
Specifications
About this item
For quantitative determination of albumin concentrations (BCP method).
- Detection range 0.3 g/dL (45 μM) to 5 g/dL (750 μM) albumin in 96-well plate assay
- The procedure involves addition of a single working reagent and incubation for five minutes
- Can readily automated for processing thousands of samples per day
Albumin is the most abundant plasma protein in human
BioAssay Systems’ BCP albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol purple that forms a colored complex specifically with albumin. The intensity of the color, measured at 610nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
It accounts for about 60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure, binding of key substances such as long-chain fatty acids, bile acids, bilirubin, haematin, calcium, magnesium. It has anti-oxidant and anticoagulant effects, and also acts as a carrier for nutritional factors and drugs, as an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition and protein-losing enteropathies. High levels are associated with dehydration. Simple, direct and automation-ready. Procedures for measuring albumin concentration in biological samples are becoming popular in Research and Drug Discovery.