About this item
The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will "compete" for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.
Type: Primary
Antigen: CD4
Clonality: Monoclonal
Clone: GK1.5
Conjugation: Biotin
Epitope:
Host: Rat
Isotype: IgG2b, kappa
Reactivity: Mouse;Golden Syrian Hamster
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Specifications
- Catalog No:
- 75875-704
- 75875-706
- Antigen name:
- CD4 molecule
- CD4 molecule
- Antibody type:
- Primary
- Primary
- Clonality:
- Monoclonal
- Monoclonal
- Clone:
- GK1.5
- GK1.5
- Host:
- Rat
- Rat
- Gene ID:
- 12504
- 12504
- Isotype:
- IgG2b kappa
- IgG2b kappa