About this item
This 5X Direct Loading buffer contains a densifying agent and a red-purple dye that allow to follow the migration of the sample during the electrophoresis.
- Highly reproducible results
- Ideal for T/A cloning
- Ultra pure enzyme
- Highly processive
Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus
Highly processive, recombinant, thermostable 5'-3' DNA polymerase catalyzes the addition of mononucleotide units to the 3'-OH end of a primer chain. Remains functional even after prolonged incubation at 95° C. This enzyme possesses 5'-3' exonucleases activity.
Quality control performed on every lot of enzymes: Specific PCR on genomic and phage templates, activity check, absence of ribonucleases, absence of nickases and endonucleases, absence of 3' exonucleases, purity on SDS-PAGE, absence of proteases.
One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74° C.
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Specifications
- Storage conditions:−20 °C
- Buffer composition:5X Direct Loading Buffer with MgCl2 solution
- Concentration:5U/μl