Malonate Broth is used for differentiating Enterobacter from Escherichia based on malonate utilization.

• Contains ammonium sulfate as a sole source of nitrogen
• Dipotassium phosphate and monopotassium phosphate provide buffering capability
• Sodium chloride maintains osmotic equilibrium
• Bromthymol blue is a pH indicator

Malonate Broth is described for differentiating Enterobacteriaceae in food and dairy products, and is suitable for organisms that would otherwise fail on the unsupplemented Leifson medium.