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Specifications
- Description:Vitamin D (25OH) Total (Rat)
- Cat. no.:102465-524
- Supplier No.:KRR1971
Specifications
About this item
Immunoenzymetric assay (ELISA) for the determination of 25-hydroxyvitamin D2 and D3 (25OH-D2 and 25OH-D3) in rat serum.
- For research use only, not for use in diagnostic procedures
- Competitive ELISA assay
- Shows very competitive sensitivity and precision
The Immunoassays Rat 25OH Vitamin D Total ELISA is a competitive ELISA assay, based on patented monoclonal antibodies, featuring our novel pre-treatment step performed inside the ELISA microtiter plate.
The assay shows very competitive sensitivity and precision and has been designed and validated specifically for rat serum samples.
The Rat 25OH Vitamin D Total ELISA is a solid phase Enzyme Linked Immunosorbent Assay performed on microtiterplates. During a first 2 hours incubation step, at room temperature, total 25OH Vitamin D (D2 and D3) present in calibrators, controls and samples is dissociated from binding serum proteins to fix on binding sites of a specific monoclonal antibody. After 1 washing step, a fixed amount of 25OH Vitamin D-labelled with biotin in presence of horseradish peroxidise (HRP), compete with unlabelled 25OH Vitamin D2 and 25OH Vitamin D3 present on the binding sites of the specific monoclonal antibody. After a 30 minutes incubation at room temperature, the microtiterplate is washed to stop the competition reaction. The Chromogenic solution (TMB) is added and incubated for 15 minutes. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is inversely proportional to the total 25OH Vitamin D (D2 and D3) concentration. A calibration curve is plotted and the total 25OH Vitamin D (D2 and D3) concentrations of the samples are determined by dose interpolation from the calibration curve.