Thioglycollate Medium without Indicator is a general-purpose medium for the cultivation of microorganisms, especially obligate anaerobes.

• Casein and soybean peptones, dextrose, and cystine supply nitrogen and carbon
• Sodium chloride provides essential ions

Casein and soybean peptones, dextrose, and cystine supply nitrogenous and carbonaceous compounds, fermentable carbohydrates, and trace ingredients. This medium is satisfactorily used as an enrichment culture for various types of specimens and also as a transportation medium.