Mycoplasma broths, when supplemented with nutritive enrichments, are used for isolating and cultivating Mycoplasma.

• Peptones and beef extract provide essential source of nitrogen
• Yeast extract is a source of vitamins and carbon
• Sodium chloride maintains the osmotic balance

Mycroplasma Broth Base without crystal violet is prepared according to the formula described by Morton and Lecci. Crystal violet is omitted from this formula due to its inhibitory action on some Mycoplasma. It has been used for the cultivation of Mycoplasma for research studies.