The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells.

• Glow-type luminescence
• Optimized for luminescence microplate readers
• Simplified data acquisition and analysis

The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells

Reporter gene assays are an important tool for biomedical and pharmaceutical researchers to monitor cellular events associated with gene expression, gene regulation, and signal transduction. Firefly luciferase, one of the most commonly used reporters, produces light by catalyzing a bioluminescence reaction that oxidizes D-luciferin in the presence of oxygen and ATP.

The application of luciferase-based reporter assay using luminescence microplate readers has become increasing popular for high-throughput analysis of gene expression as well as drug discovery applications. The SpectraMax Glo Steady-Luc Reporter Assay Kit is designed for optimal performance on microplate readers with high throughput enabled by both the microplate format and assay signal stability.

The assay kit is optimized for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.