K. pneumoniae Recombinant Kanamycin kinase type II (from E. coli)
75791-878
: Prosci
:
- Enzyme type:Recombinant
- Source:E. coli
- Species:K. pneumoniae
- Size:0.05 mg
- Storage conditions:Lyophilized protein should be stored at –20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4...7 °C for 2 - 7 days. Aliquots of reconstituted samples are stable at –20 °C for 3 months.
- Endotoxin content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Enzyme name:Kanamycin kinase type II
- Enzyme synonyms:Aminoglycoside 3'-phosphotransferase, APH(3')-II, APH(3')II, Kanamycin kinase type II, Neomycin-kanamycin phosphotransferase type II, neo
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:29 kD
- Sequence:Met1-Phe264
- Formulation:Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Cat. no.:75791-878
- Supplier No.:92-658
Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria
This recombinant protein can be used for biological assays. For research use only.
These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.