Human Recombinant Isocitrate Dehydrogenase (from E. coli)
75791-788
: Prosci
:
- Enzyme type:Recombinant
- Source:E. coli
- Species:Human
- Size:0.05 mg
- Tag sequence:C-8 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Endotoxin content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Enzyme name:Isocitrate Dehydrogenase
- Enzyme synonyms:Isocitrate Dehydrogenase [NADP] Cytoplasmic, IDH, Cytosolic NADP-Isocitrate Dehydrogenase, IDP, NADP(+)-Specific ICDH, Oxalosuccinate Decarboxylase, IDH1, PICD
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:48.1 kD
- Sequence:Met1-Leu414
- Formulation:Supplied as a 0.2 µm filtered solution of 50 mM Tris, 200 mM NaCl, 10% glycerol, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Cat. no.:75791-788
- Supplier No.:92-613
Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family
This recombinant protein can be used for biological assays. For research use only.
Fusion-Tag: C-8 His tag
IDH1 exists as a homodimer, binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid.