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Human Recombinant PAPS synthase 1 (from Cells)
Catalog # 75791-320
Supplier:  Prosci
CAS Number:  
Human Recombinant PAPS synthase 1 (from Cells)
Catalog # 75791-320
Supplier:  Prosci
Supplier Number:  92-367
CAS Number:  

Specifications

  • Enzyme type:
    Recombinant
  • Source:
    Cells
  • Species:
    Human
  • Size:
    0.05 mg
  • Tag sequence:
    C-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Endotoxin content:
    <0.1 ng/ug (1 IEU/ug) as determined by LAL test.
  • Enzyme name:
    PAPS synthase 1
  • Enzyme synonyms:
    Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, PAPS synthase 1, PAPSS 1, Sulfurylase kinase 1, SK 1, ATPSK1, PAPSS, PAPSS1
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    71.9 kD
  • Sequence:
    Met1-Ala624
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl and 20% glycerol. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    Biological assays
  • Cat. no.:
    75791-320
  • Supplier No.:
    92-367

Specifications

About this item

PAPSS1 is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity

This recombinant protein can be used for biological assays. For research use only.

Fusion-Tag: C-6 His tag

In the N-terminal section, it belongs to the APS kinase family; while the C-terminal section belongs to the sulfate adenylyltransferase family. PAPSS1 can be inhibited by chlorate, and is expressed in many tissues, such as high endothelial venules (HEV) cells and in cartilage. PAPSS1 mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate. In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. PAPSS1 also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.