Human Recombinant GRB2 (from E. coli)
75788-674
: Prosci
:
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Human
- Size:0.05 mg
- Tag sequence:C-6 His tag
- Storage conditions:Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
- Endotoxin content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Protein synonyms:SH2/SH3 Adapter GRB2|Growth Factor Receptor-Bound Protein 2|Adapter Protein GRB2|Protein Ash|GRB2|ASH
- Protein/peptide name:GRB2
- Purity:> 95% as determined by reducing SDS-PAGE
- Molecular weight:26.3 kD
- Sequence:Met1-Val217
- Formulation:Lyophilized from a 0.2 um filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
- Cat. no.:75788-674
- Supplier No.:92-001
As an adaptor protein, Growth Factor Receptor-Bound Protein 2 (GRB2) is involved in siganl transduction and consists of a central SH2 domain flanked by two SH3 domains. GRB2 associates with activated Tyr-phosphorylated EGF receptor/EGFR and PDGF receptors via its SH2 domain, stimulating GTP binding to Ras, which in turn activates MAPK and other signaling pathway.It also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK. probably via the concerted action of both its SH2 and SH3 domains.
- Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.