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Human Recombinant SEMA5A (from Cells)
Human Recombinant SEMA5A (from Cells)
Catalog # 75789-578
Supplier:  Prosci
CAS Number:  
Human Recombinant SEMA5A (from Cells)
Catalog # 75789-578
Supplier:  Prosci
Supplier Number:  91-451
CAS Number:  

Specifications

  • Protein/peptide type:
    Recombinant
  • Source:
    Cells
  • Species:
    Human
  • Size:
    0.05 mg
  • Tag sequence:
    C-6 His tag
  • Storage conditions:
    Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
  • Endotoxin content:
    <0.1 ng/ug (1 IEU/ug) as determined by LAL test.
  • Protein synonyms:
    Semaphorin-F|SEMAF|SEMA5A|Semaphorin-5A|Sema F
  • Protein/peptide name:
    SEMA5A
  • Purity:
    > 95% as determined by reducing SDS-PAGE
  • Molecular weight:
    84.71 kD
  • Sequence:
    Glu23-Thr765
  • Formulation:
    Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, 0.05% Tween 20, pH 7.2. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    For Biological Assays
  • Cat. no.:
    75789-578
  • Supplier No.:
    91-451

Specifications

About this item

Semaphorin-5A (SEMA5A) is a member of the Semaphorin family of axon guidance molecules. SEMA5A is a 140 kDa protein. Class 5 Semaphorins are type I transmembrane glycoproteins with an N- terminal Sema domain and multiple juxtamembrane type 1 Thrombospondin (TSP) repeats within their extracellular domains. SEMA5A is expressed in neuroepithelial cells surrounding retinal axons, oligodendrocytes, the base of limb buds, the mesoderm surrounding cranial vessels , and the cardiac atrial septum and endocardial cushions, Human SEMA5A cDNA encodes a signal sequence, a extracellular domain (ECD), a transmembrane sequence and an cytoplasmic portion. SEMA5A mutations have been implicated in the genetic syndrome,cri-du-chat,while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson. The expression of SEMA5A may be upregulated in metastatic cancer cells and downregulated in autism.

  • Fusion-Tag: C-6 His tag

This recombinant protein can be used for biological assays. For research use only.