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Human Recombinant Carbonic Anhydrase 5B (from E. coli)
Human Recombinant Carbonic Anhydrase 5B (from E. coli)
  75788-910
 :  Prosci
 :  
Human Recombinant Carbonic Anhydrase 5B (from E. coli)
  75788-910
 :  Prosci
 :  91-093
 :  

 

  • Enzyme type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Size:
    0.05 mg
  • Tag sequence:
    C-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Endotoxin content:
    <0.1 ng/ug (1 IEU/ug) as determined by LAL test.
  • Enzyme name:
    Carbonic Anhydrase 5B
  • Enzyme synonyms:
    Carbonic Anhydrase 5B Mitochondrial, Carbonate Dehydratase VB, Carbonic Anhydrase VB, CA-VB, CA5B
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    33.77 kD
  • Sequence:
    Cys34-Pro317
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    Bioassay
  • Cat. no.:
    75788-910
  • Supplier No.:
    91-093

 

 

Carbonic Anhydrase 5B (CA5B) is a member of alpha-carbonic anhydrase family (CAs) that catalyze the reversible hydration of carbon dioxide

This recombinant protein can be used for biological assays. For research use only.

Fusion-Tag: C-6 His tag

CAs is associated with many biological processes, including calcification, respiration, bone resorption, acid-base balance and the formation of aqueous humor. CA5B is highly expressed in heart, pancreas, kidney, placenta, lung, and skeletal muscle, but it is restricted to the liver. CA5B is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA-VA. CA5B is inhibited by coumarins, sulfonamide derivatives such as acetazolamide (AZA), saccharin, and Foscarnet.