Buffers and Accessories, Biotium
About this item
Commonly used buffers and accessories for microbiology, cell biology and microscopy.
- Ready-to-use buffers formulated for immunofluorescence staining of intracellular antigens for analysis by flow cytometry
- Fish gelatin blocking reagent for Western blotting and immunostaining
- Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes
- Dilute solutions of antibody
- Blocking agent to reduce non-specific binding
Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Fish gelatin can be used in blocking buffers to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
Ultrafiltration vials with MWCO=10K are recommended for ultrafiltration of antibodies and other proteins larger than 30 kDa. Vials with MWCO=3K can be used for ultrafiltration of proteins larger than 10 kDa. Ultrafiltration can be used to rapidly and easily remove small molecules such dyes, biotin, Tris, glycerol and glycine from antibody solutions, or to concentrate antibodies prior to conjugation.
Activated Charcoal Decontamination Bags retain ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.
Mini glass tubes offer higher sensitivity than plastic tubes when used with Biotium’s AccuLite™ Mini-Fluorometers.
10 Options Available Below
Product Details & Documents
Commonly used buffers and accessories for microbiology, cell biology and microscopy.
- Ready-to-use buffers formulated for immunofluorescence staining of intracellular antigens for analysis by flow cytometry
- Fish gelatin blocking reagent for Western blotting and immunostaining
- Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes
- Dilute solutions of antibody
- Blocking agent to reduce non-specific binding
Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Fish gelatin can be used in blocking buffers to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
Ultrafiltration vials with MWCO=10K are recommended for ultrafiltration of antibodies and other proteins larger than 30 kDa. Vials with MWCO=3K can be used for ultrafiltration of proteins larger than 10 kDa. Ultrafiltration can be used to rapidly and easily remove small molecules such dyes, biotin, Tris, glycerol and glycine from antibody solutions, or to concentrate antibodies prior to conjugation.
Activated Charcoal Decontamination Bags retain ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.
Mini glass tubes offer higher sensitivity than plastic tubes when used with Biotium’s AccuLite™ Mini-Fluorometers.
Documents
Certificates
Search by Lot NumberBiotium TrueBlack® Reagents for Immunofluorescence and Western Blots
Product Literature
Product Family Options
Product Information
- SizeAvailabilityPrice
- 89411-554Flow Cytometry Fixation/Permeabilization KitSupplier Number: 23006Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 89410-740Fish Gelatin PowderSupplier Number: 22011Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 89410-744Bovine Serum Albumin Fraction VSupplier Number: 22013Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 89411-09610X Fish Gelatin Blocking AgentSupplier Number: 22010Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 89411-098Bovine Serum Albumin 30% solutionSupplier Number: 22014Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 89411-100Ready-to-use Formaldehyde-based Fixation BufferSupplier Number: 22015Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 89411-102Cell Permeabilization BufferSupplier Number: 22016Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 89427-054Ultrafiltration Vial, MWCO=10KSupplier Number: 22004
- 89493-026Ultrafiltration Vial, MWCO=3KSupplier Number: 22018
- 89493-028Mini Glass Tubes (250 uL capacity)Supplier Number: 22019
Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/ca/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Recommendations will be personalized based on your shopping preferences only if you have given your consent by enabling "Enhance my Shopping Experience" on the "Personal Info page".
Otherwise, you will receive generic recommendations.
Catalog #: CA21905-026
Supplier: Kimberly-Clark