Accutase® is a cell detachment solution containing proteolytic and collagenolytic enzymes.

This solution is a replacement for Trypsin-EDTA solution and is useful for the detachment of cells from standard tissue culture plasticware and adhesion coated plasticware and polymers

Cell lines tested for Accutase™ application include fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and HNK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, MIC5, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251, D54, HT1080 fibrosarcoma cells, HT29 colon cancer cells, Sf9 insect cells, human embryonic stem cells, human mesenchymal stem cells and human neural stem cells.

It is a replacement for Trypsin-EDTA solution and is useful for the detachment of cells from standard tissue culture plasticware and adhesion coated plasticware and polymers.