Pierce™ Ni-NTA chromatography cartridges are convenient, ready to use pre-packed devices for the immobilized metal affinity chromatography purification of polyhistidine-tagged proteins from a soluble protein extract.

• High capacity which can bind up to 60 mg of 6xHis-tagged protein per ml of resin
• Purify proteins using native or denaturing conditions
• Compatible to use with other cell lysis reagents and a variety of buffer additives
• Same batch of resin can be reused at least five times with no loss in performance
• Easy to use - pre-formulated buffers available for kit formats
• Available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates

HisPur Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates, such as those that are attained with Thermo Scientific B-PER bacterial protein extraction reagents.

These cartridges contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins. The resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates and performs well in batch-binding and spin-column procedures at a variety of scales.

The resin performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular Ni-NTA resins from other suppliers. HisPur Ni-NTA Resin is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.