Pierce™ HisPur™ cobalt chromatography cartridges are convenient, ready-to-use pre-packed devices for efficient purification of polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells.

• Binds at least 10 mg of purified 6 x His-tagged protein per ml of resin
• Cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
• Low metal leaching means no metal contamination in eluted histidine-tagged protein sample

HisPur™ cobalt chromatography cartridges are compatible with the major automated liquid-chromatography systems or manual syringe processing. The cartridges attach directly to ÄKTA™ or FPLC Systems without additional connectors. The included accessory pack readily adapts cartridges for use with Luer-Lok® syringe fittings or ¹/₁₆" tubing. These cartridges enable fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses.

His-tagged proteins are purified from total soluble protein extract using a cobalt-charged tetradentate chelator immobilized onto 6% crosslinked agarose.