The Thermo Scientific Pierce Classic Magnetic IP/Co-IP Kit enables highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of antigens and protein-complexes using less than 10 µg of antibody and a magnetic separator.

• Magnetic Beads based on Protein A/G Recombinant Protein EnsuresCompatibility with Most Primary Antibodies, Whether from Mouse or Rabbit
• Immunoprecipitate in as few as 30 Minutes to Reduce Background and Improve the Capture of Transient Protein Complexes
• Immobilize your Antibody to Prevent Contamination in your Eluate
• No Leaching of Protein A/G in the Presence of Detergents, Low pH Buffers or Common Mass Spectrometry Solvents
• Immunoprecipitate with Half the Recommended Volume of Magnetic Particles Compared to other Magnetic Beads
• The Comprehensive Co-IP Kit Contains the Magnetic Beads and all Essential Buffers to Complete Immunoprecipitation Experiments

This immunoprecipitation kit uses high-quality Thermo Scientific Pierce Protein A/G Magnetic Beads and optimized buffers and flexible protocol to accomplish high-yield IP or co-IP with manual or automated magnetic-separation tools

Includes:
Pierce Protein A/G Magnetic Beads, lysis/wash buffer, elution buffer, neutralization buffer and sample loading buffer

Immunoprecipitation with magnetic particles is performed much like IP with beaded agarose, except that separations are performed using a magnet rather than by centrifugation. The specific antibody is first added to the sample to form an immune complex that is then bound to the magnetic beads. The complex is washed to remove non-bound material and a low-pH elution buffer dissociates the bound immune complex from the Protein A/G. Alternatively, the Lane Marker Sample Buffer is included for dissociation using denaturing conditions or for downstream sample prep for SDS-PAGE analysis.

The optimized Lysis/Wash Buffer optimizes yield and efficient binding of antibody-antigen and co-IP interactions. The relatively gentle, low-pH Elution Buffer dissociates the bound immune complex from the Protein A/G. Alternatively, the included Lane Marker Sample Buffer provides rapid, denaturing elution for direct SDS-PAGE analysis of IP products.