About this item
Thermo Scientific SulfoLink Immobilization Kit for Proteins is a kit containing all the reagents for preparing the protein sample for efficient immobilization and five columns containing SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.
- Specific to sulfhydryl (-SH) groups
- Fast—prepare and couple protein samples in 3.5 hours
- Flexible coupling conditions, use pH 7.5 to 9.0 aqueous buffers, organic solvent or denaturant
- Streamlined protocols for sample preparation, immobilization, and affinity purification
- High capacity—Immobilize 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin
When incubated with a solution of protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the protein to the resin
Applications:
Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.