Pierce™ AminoLink™ Plus Immobilization Kit, 2 ml, Thermo Scientific

Supplier: Thermo Scientific

Certificates

Supplier: Thermo Scientific

Certificates

About this item

The AminoLink Plus Immobilization Kit contains AminoLink Plus Coupling Resin and additional reagents needed to perform 5 protein immobilizations, with expected yields of 2 to 40 mg protein per column.

Aldehyde-activated crosslinked 4% beaded agarose
Immobilize molecules via primary amines (-NH₂)
Efficient coupling over a wide range of pH (4 to 10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent)
Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
Versatile and reusable

The coupling resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Delivery: Kit contains AminoLink™ Plus columns, 2 mL (5 columns), PBS pack (makes 500 mL), citrate-carbonate buffer pack (makes 500 mL), sodium cyanoborohydride solution, 5 M (0.5 mL), quenching buffer (60 mL), wash solution (240 mL), and column accessories.

Caution: For Research Use Only. Not for use in diagnostic procedures.

Specifications

Storage conditions:
Store at 4˚C
Sufficient for:
Immobilizing 2 to 40 mg of protein per column
Contents:
AminoLink Plus Column, 2mL (1 or 5 columns)
PBS Pack (makes 500 mL), 1 pack
Citrate-Carbonate Buffer Pack (makes 500 mL), 1 pack
Sodium Cyanoborohydride Solution, 5M, 0.5 mL
Quenching Buffer, 15 mL
Wash Solution, 50 mL
Column Accessories