The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.

• Sensitive—highly sensitive detection of firefly luciferase activity
• Odorless—reagents have no foul or toxic odor, eliminating the need to work in a fume hood
• Convenient—one-step, mix-and-read protocol; no additional reagents required
• Stable—increased signal half-life and stability compared to flash assays
• Simple—does not require a luminometer with injectors
• Automation-friendly—amenable to high throughput assays

Firefly luciferase bioluminescence provides researchers with a non-radioactive assay for measuring transcriptional activity of regulatory elements. Firefly luciferase reporter activity can be used to determine promoter activity, to study upstream pathway activity in the cell, or to determine effect of activators or inhibitors.