About this item
Thermo Scientific EZ-Link™ Desthiobiotinylation and Pull-down Kit is a complete set of reagents to biotin-label purified proteins and use them to purify interaction complexes using streptavidin agarose resin with mild elution conditions.
- Sulpho-NHS-LC-Desthiobiotin label proteins with a biotin-like that specifically binds streptavidin
- Simple, optimized protocol and reagents to label proteins
- Recover (purify) intact, functional protein interaction complexes
- PEG groups in spacer arms enhance water solubility of biotinylated molecules and help prevent aggregation of biotinylated antibodies
EZ-Link™ Desthiobiotinylation and Pull-down Kit uses Sulpho-NHSLC-Desthiobiotin reagents to label target proteins on primary amines, desalting columns to clean up the labeling reaction, and immobilized high capacity streptavidin resin and buffers to perform pull-down assays
Pull-down assays use the tagged or labelled bait protein coupled to resin to capture a prey protein contained in a cell lysate or other unpurified protein mixtures. This technique can identify novel interactions between a known protein (bait) and previously undiscovered target (prey) or it can confirm the interaction between a known (bait) and a known target (prey) protein.
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