Human Recombinant IL6 (from E. coli)

IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant Human IL-6 is a 20.9 kDa protein containing 184 amino acid residues.Manufactured using all Animal-Free reagents.

Carrier free
Animal-free

PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

Verified by N-terminal and Mass Spectrometry analyses (when applicable).

: For research use only. Not for use in diagnostic or therapeutic procedures.

Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Storage conditions:
−20 °C
Endotoxin content:
<0.1 ng/μg of protein (<1 EU/μg).
Biological activity:
The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of the IL-6 dependent murine 7TD1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.
Animal-free:
Y
Protein/peptide name:
IL6
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Formulation:
Lyophilized
Shipping temperature:
RT