You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Pierce EZ-Link® Carboxyl Reactive Biotinylation Reagents, Thermo Scientific
Supplier: Thermo Scientific
Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatized biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4.5 - 5 and requires just minutes to complete.
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Pierce™ ABC Peroxidase Standard Staining Kit, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.
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Pierce™ PEGylated SPDP Crosslinkers, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Pierce™ PEGn-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a n-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.
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Pierce™ FITC Antibody Labeling Kit, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ FITC Antibody Labeling Kit is a multi-component kit containing a high performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.
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CLIP-CELL® 505 , New England Biolabs
Supplier: New England Biolabs (NEB)
CLIP-Cell™ 505 is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro
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SNAP-SURFACE® 594, New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Surface® 594 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living or fixed cells
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Pierce™ Fluorescence Biotin Quantitation Kit, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific™ Pierce Fluorescence Biotin Quantitation Kit requires only ten microliters of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.
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SNAP-CELL® 647-SiR, New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Cell 647-SiR is a far-red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro
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femtoCHROMO™- AP Kit for Chromogenic Detection, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' femtoCHROMO™- AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining
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Pierce™ Controlled Protein-Protein Cross-Linking Kit, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.They insert an unbreakable molecular bridge between two proteins with easy-to-control stepwise coupling.
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Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers, Thermo Scientific
Supplier: Thermo Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.
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Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific
Supplier: Thermo Scientific
Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.
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Pierce™ Immunoprecipitation, Crosslink IP Kit, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.
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DyLight™ Antibody Labeling, Long Stokes Shift Dyes, Thermo Scientific
Supplier: Thermo Scientific
The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 - 145 nm in excitation/emission wavelengths.
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femtoCHROMO™- HRP Kit for Chromogenic Detection, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining
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SDC™ (Steroid/Drug/Compound) Immobilization Kit, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' SDC™ (Steroid/ Drug/ Compound) Immobilization kit is designed for the immobilization of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification
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HOOK™ Activated Agarose (Amine Reactive) for Affinity Column Generation, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups
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Pierce™ PEGylated BM (bismaleimido) Crosslinkers, Thermo Scientific
Supplier: Thermo Scientific
Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions
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Pierce™ GlycoLink™ Coupling Catalyst, Thermo Scientific
Supplier: Thermo Scientific
An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.
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Pierce™ CA(PEG)n Carboxy-PEG-Amine Compound, Thermo Scientific
Supplier: Thermo Scientific
The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers
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CLIP-Biotin, New England Biolabs
Supplier: New England Biolabs (NEB)
CLIP-Biotin is a cell-permeable substrate (BC-Biotin) based on biotin with an amidocaproyl linker
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Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific
Supplier: Thermo Scientific
DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.