Protein A Sepharose™ CL-4B Affinity Chromatography Media, Cytiva

: Cytiva

Protein A Sepharose™ CL-4B Affinity Chromatography Media, Cytiva

: Cytiva

Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain.

For affinity chromatography
For immunoglobin purification and fractionation, immunoprecipitation procedures and polyclonal antibody purification
Strong binding to Fc region of IgG
High capacity - 20 mg human IgG/ml
Cyanogen bromide activation coupling method
Stable in all commonly used aqueous buffers, 6 M guanidine-HCl, 8 M urea
Freeze-dried powder or preswollen in 20% ethanol
Negligible volume variation due to changes in pH or ionic strength

The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.

pH range:
3 - 9
Matrix:
Cross-linked agarose, 4%
Ligand:
Native Protein A
Storage temperature:
2 - 8 °C
Binding capacity:
≈ 20 mg human lgG/mL medium
Flow rate:
<150 cm/h