You searched for: Protein Linking and Labeling Systems

Supplier: G-Biosciences

G-Biosciences' HOOK™ BiotinQuant is designed for the estimation of biotin conjugation.

Supplier: Thermo Scientific

Activated peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins.

Supplier: G-Biosciences

Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.

Supplier: New England Biolabs (NEB)

SNAP-Cell® Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag® in solution or in living cells

Supplier: Thermo Scientific

Pierce™ NHS-Fluorescein Antibody Labeling Kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Supplier: G-Biosciences

G-Biosciences' avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates

Supplier: Thermo Scientific

Thermo Scientific DyLight Fluor Phosphine Reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.

Supplier: Thermo Scientific

Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (biotin-N4-CTP) onto the 3' end of DNA strands where the label will be less likely to interfere with hybridization or sequence-specific binding of proteins.

Supplier: New England Biolabs (NEB)

SNAP-Surface® Alexa Fluor® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells

Supplier: New England Biolabs (NEB)

SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells

Supplier: Thermo Scientific

The Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit provides researchers with a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait.

Supplier: New England Biolabs (NEB)

SNAP-Cell® TMR-Star is a red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro

Supplier: Thermo Scientific

Thermo Scientific Pierce Maleimide Activated Horseradish Peroxidase is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

Supplier: New England Biolabs (NEB)

CLIP-Cell™ TMR-Star is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro

Supplier: Thermo Scientific

Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide

Supplier: G-Biosciences

G-Biosciences' recombinant streptavidin comes from streptomyces avidinii, produced in E.coli

Supplier: G-Biosciences

G-Biosciences' Carbohydrate Coupling Resin is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups

Supplier: New England Biolabs (NEB)

The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the target protein from the affinity tag.

Supplier: New England Biolabs (NEB)

CLIP-Cell™ 505 is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro

Supplier: Thermo Scientific

Thermo Scientific™ Pierce Fluorescence Biotin Quantitation Kit requires only ten microliters of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

Supplier: Thermo Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: New England Biolabs (NEB)

A 52.8 kDa non-glycosylated protein with a very high affinity for biotin

Supplier: New England Biolabs (NEB)

SNAP-Biotin®i s a cell-permeable substrate (BG-Biotin) based on biotin with an amidocaproyl linker

Supplier: Thermo Scientific

Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.

Supplier: G-Biosciences

G-Biosciences' HOOK™ Peptide Coupling (Amine Reactive) Kit is designed for the coupling of peptides to carrier proteins, utilizing the primary amines and carboxyl groups of the peptides and carrier proteins

Supplier: Thermo Scientific

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatized biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4.5 - 5 and requires just minutes to complete.

Supplier: Rockland Immunochemical

Supplier: Thermo Scientific

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

Supplier: Thermo Scientific

Eliminate soluble components and reduce disulfide bonds without sacrificing valuable protein or peptide samples.

Supplier: G-Biosciences

The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.