PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium.

• Extracts proteins from E. coli cells
• Buffered mixture of concentrated detergents
• Protects protein from the pH extremes produced in high density culture media

The PopCulture protein purification procedure is ideally suited to high throughput (HT) robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Using this method, cell culture, protein extraction and purification performed in the original culture tube or multiwell plate. PopCulture perforates the E. coli cell wall without denaturing soluble protein and protects protein from the pH extremes produced in high density culture media. Recombinant proteins can be assayed directly or purified by adding an affinity matrix, washing the matrix: target protein complex to remove culture medium and cellular contaminants and eluting the purified protein from the matrix.

To further enhance the PopCulture purification procedure, lysozyme and/or Benzonase® Nuclease may be added.